Thymogen
Also known as: Thymagen, EW, Glu-Trp, L-Glu-L-Trp, Oglufanide, Timogen, IM-862
EW Dipeptide | Thymus Immune Bioregulator
Overview
Thymogen (EW dipeptide) is a Khavinson bioregulator consisting of glutamic acid and tryptophan, originally isolated from calf thymus extracts (Thymalin) in the late 1980s. Developed by Professor Vladimir Khavinson, it has been registered in Russia since 1990 in multiple forms including injectable solution, nasal spray, and topical cream. Thymogen modulates both humoral and cellular immunity, activates T-cell differentiation, and has demonstrated geroprotective (anti-aging) and antitumor activities in research studies.
Key benefits
- Modulates humoral and cellular immunity
- Activates T-cell differentiation
- Normalizes T-lymphocyte populations (CD3+, CD4+, CD8+)
- Stimulates immunoglobulin production
- Enhances regeneration and cellular metabolism
- Demonstrated geroprotective (anti-aging) activity
- Antitumor activity in research models
- Registered pharmaceutical in Russia
- Multiple delivery forms available
Mechanism of action
Thymogen works through multiple immunomodulatory mechanisms: (1) activates T-cell differentiation and T-cell recognition of peptide-MHC complexes, (2) induces changes in intracellular cyclic nucleotide composition, (3) activates neutrophilic chemotaxis and phagocytosis, (4) normalizes T-lymphocyte concentrations and ratios (CD3+, CD4+, CD8+), (5) stimulates production of immunoglobulins (IgA, IgG, IgE, IgM), and (6) enhances lymphocyte differentiation receptor expression. Research suggests Thymogen may interact specifically with the AACG DNA sequence, affecting gene expression. The peptide is rapidly distributed to thymus, lymph nodes, liver, adrenals, and kidneys.
Molecular data
- Type
- Dipeptide bioregulator
- Half-life
- Not established
Sequence
Glu-Trp (EW)
Indications
What the research community uses this compound for, with self-reported effectiveness.
Immune Support
Immune System Modulation
Most effectiveModulates both humoral and cellular immune responses for balanced immunity.
T-Cell Function
EffectiveActivates T-cell differentiation and normalizes CD3+, CD4+, CD8+ populations.
Immunoglobulin Production
EffectiveStimulates production of IgA, IgG, IgE, and IgM antibodies.
Post-Operative Recovery
ModerateStudied for improving surgical outcomes through immune support.
Anti-Aging Research
Geroprotection
EffectiveDemonstrated geroprotector activity in aging research.
Lifespan Extension
EffectiveIn combination with pineal peptides, showed significant mean lifespan increases in animal models.
Antitumor Activity
ModerateInhibits spontaneous carcinogenesis and shows robust antitumor effects in research.
Clinical Research Areas
Cancer Support
ModerateStudied in Russia for regulating immune response in oncology patients.
Heart Disease
EmergingResearch interest in cardiovascular applications.
Diabetes Support
EmergingInvestigated for metabolic and immune aspects of diabetes.
Dosing protocols
Common protocols by delivery method. Adjust the curve below to model accumulation in your own cycle.
Injectable
Injectable form for intramuscular or subcutaneous administration. This is the original delivery method registered in Russia. Typical protocols involve 10-20 day cycles repeated 2-3 times per year.
| Protocol | Dose | Frequency | Route / duration |
|---|---|---|---|
| Standard immune support | 10-20 mg | Daily for 10-20 days | IM or SubQ |
| Maintenance protocol | 20 mg | 2-3 cycles per year | IM or SubQ |
Preparation
You will need
- Bacteriostatic water
- Insulin syringes
- Alcohol swabs
- Sterile vial
Steps
- Clean work area and hands
- Reconstitute with bacteriostatic water
- Inject slowly down vial wall
- Gently swirl until dissolved
- Store refrigerated at 2-8°C
- Use within 4 weeks
Work out the water volume and syringe units in the reconstitution calculator →
Nasal
Metered-dose nasal spray form registered in Russia. Convenient for non-injection administration with good absorption through nasal mucosa.
| Protocol | Dose | Frequency | Route / duration |
|---|---|---|---|
| Nasal administration | As per product directions | Daily during cycle | Intranasal spray |
Topical
0.05% cream formulation registered in Russia for external use. Applied to skin for local effects.
| Protocol | Dose | Frequency | Route / duration |
|---|---|---|---|
| Topical use | Apply thin layer | As directed | Topical cream |
Interactions
Pairwise interactions with other compounds you might be cycling — the same data behind the cycle builder's safety check.
Epitalon
SynergisticCombination showed significant lifespan increases and robust antitumor activity in research.
Thymalin
SynergisticThymogen was isolated from Thymalin; they share complementary thymus-supporting mechanisms.
Vilon
SynergisticBoth are Khavinson thymus dipeptides; often combined in comprehensive immune protocols.
Thymosin Alpha-1
CompatibleDifferent thymic peptides with complementary immune mechanisms.
Thymulin
CompatibleDifferent structures and mechanisms; can be used together.
Timeline
Typical week-by-week effects reported in clinical and observational data.
- Hours
Rapid tissue distribution to thymus, lymph nodes, liver, kidneys
- Days 1-10
T-cell activation and differentiation begins
- During cycle
Immunoglobulin production stimulated; immune markers improve
- Post-cycle
Effects persist due to cellular and epigenetic changes
- Long-term
Cumulative geroprotective benefits with regular cycling
Safety
Reported adverse effects, contraindications, and what to monitor on cycle.
Common side effects
- Generally well-tolerated
- Injection site reactions (mild)
- Minimal side effects reported in clinical use
Rare side effects
- Allergic reactions (rare)
Stop signs — stop and seek advice
- Signs of allergic reaction
- Unusual immune symptoms
- Severe injection site reactions
Contraindications
- Active autoimmune diseases (use with caution)
- Organ transplant recipients on immunosuppression
- Known hypersensitivity to component amino acids
- Pregnancy or breastfeeding
Monitoring
- Immune function if testing available
- T-cell subsets for research protocols
- General well-being
Quality checks
What a legitimate product looks like, what should give pause, and what should make you stop.
Good signs
- White to off-white lyophilized powder
- Clear solution after reconstitution
- Certificate of analysis with purity verification
- Proper packaging and labeling
Warning signs
- Unknown source or unverified purity
Stop signs — don't use
- Discolored powder
- Cloudy or particulate solution
- Damaged packaging or broken seal
References
Primary literature and clinical-trial data informing this entry.
Immunomodulatory synthetic dipeptide L-Glu-L-Trp slows down aging and inhibits spontaneous carcinogenesis in rats
Khavinson VKh, Morozov VG · Biogerontology · 2001
Thymogen demonstrated geroprotector activity and inhibited spontaneous carcinogenesis in aging rat models.
Natural and synthetic thymic peptides as therapeutics for immune dysfunction
Journal of Immunopharmacology · 1997
Review of thymic peptide therapeutics including Thymogen for immune modulation.
Peptide Bioregulators from Thymus and Pineal Gland
Khavinson VKh et al. · Biogerontology · 2003
Significant lifespan increases and antitumor activity with combined Thymogen and Epitalon treatment.
Thymogen: Mechanism of Action Studies
Russian Medical Research
Thymogen activates T-cell differentiation, modulates cyclic nucleotides, and may interact with AACG DNA sequences.
Last updated . How this page was sourced is described in the methodology.